Isolation and Characterization f Pichia jadinii MitochondrialNucleoids from the Yeast
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چکیده
Mitochondrial (mt) nucleoids were isolated with a high degree of purity from the yeast Pichia judinii, in which the mitochondrial DNA (mtDNA) is linear. Field-inversion gel electrophoresis (FIGE) reyealed that significant amounts of mtDNA could be isolated intact, as linear molecules of 41 kbp, from the isolated mt-nucleoids. Fifteen different proteins were detected in the mt-nucleoid fraction and, eight
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